WELCOME TO UCSD LABORATORY SERVICES GUIDE

 

 

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GENERAL LABORATORY NOTES for IMMUNOGENETICS & TRANSPLANTATION


General Sample Requirements:

     Each blood tube or individual container must be labeled with:

          - Patient's legal full name

          - At least one of the following identifiers:

                    Medical Record Number   

                    Date of Birth

                    Social Security Number

                    HIC Number

                    Reference ID Number

          - Draw Date / Time

          - Phlebotomist signature

          - Vials containing lymph nodes, spleen, or other tissue must also be individually labeled.

 

All samples must be maintained at room temperature.  Do not refrigerate.  All samples must be delivered to the lab as soon as possible.  Cell surface markers on day of collection.

 

 

***SPECIMEN REQUIREMENTS:

 

Yellow Top (ACD-A) Tubes (10 ml):

     - Avoid hemolysis.

     -  Keep at room temperature - Do not refrigerate.

     - Mix gently but thoroughly.

     - Tubes may be obtained from ITL.  Call lab to request.

     - Bone Marrow specimens must be mixed well after aspiration. 

     - Samples for Immunophenotyping by Flow Cytometry must be received in lab on the day of collection.

 

GREEN TOPS (Heparin):

     - Samples for Cell Surface Makers must be received in lab on the day of collection.

 

BONE MARROW SPECIMENS:

     - Mix well after aspiration.

 

BIOPSIES/TISSUES (Lymph Nodes, Spleen):

     - Preserve in RPMI medium with HEPES buffer.

     -  maintain at room temperature