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  Schlichter Test Specimen Type:
  also known as MBD , Maximum Bactericidal Dilution, Serum Killing Test, Schlichter

Blood


MICROBIOLOGY

Specimen requirements: 7 mL Red Stopper; Plain
Unit of measure: Highest dilution of serum which kills the disease microbe.
Ideal sample: 10mL
Absolute minimum amount: 5 mL (3 mL if pediatric)
Method of collection: Use aeseptic technique used for collecting blood cultures.
Transportation: Must be submitted to laboratory within 1 hour of collection.
Label instructions: All containers must be labeled with patient name and medical record number, date/time sample collected.
Request test on form: Multi Labs/Micro/Viro/Serology ( 151-905 )
Order/entry screen(s): Microbiology/Virology
Stat frequency: N/A
Stat turn around time: N/A
Routine frequency: Daily
Must be in by: Noon
Routine turn around time: 48 to 72 hours
For Hillcrest deliver to: Microbiology : Room 2-105
For Thornton deliver to: Microbiology : Processing Desk, 1st floor,
  Hospital, room 1-013
Scheduling requirements : All requests for MBC determinations must be approved by the attending infectious disease specialist.
Method: N/A
Special Instructions: Patient's infecting organism needs to be saved in order to perform this test. Most isolates at least one week from date of culture. If the isolate has not been saved, the procedure cannot be done. The ordering physician must specify the collection date (or culture number), culture site, time of antibiotic dose (peak or trough), and pathogen to be tested. Two serum samples are usually collected for performing the serum killing test (schlichter). The trough is obtained just before the next dose of antibiotic; the peak is obtained anywhere from 15 minutes to 1 hour immediately following the antibiotic dose, time dependant on method of dose. Results: MID (maximum inhibitory dilution) is expressed as the highest dilution of patient's serum that will inhibit growth of a specific pathogen isolated from that patient. MBD (maximum inhibitory dilution) is expressed as the highest dilution of patient's serum that kills the organism. Contraindications: Organisms that are too fastidious to grow in broth media will not be tested for serum killing test.
  Results are available on a Chart Copy
  Results are Quantitative
CyberLAB abbreviation: SCHLC
CyberLAB code: 4995
Lab Processing Instructions: N/A