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  Heparin-Platelet Factor 4 Ab Assay (ELISA) Specimen Type:
  also known as Heparin-PF4 ELISA, PF4-ELISA

Blood


SPECIAL COAGULATION LAB

Specimen requirements: Red Stopper, Plain
Reference ranges:
CATEGORY LOW   HIGH   UNITS   QUALITATIVE  
N/A N/A N/A N/A < 0.400
Critical value:
CATEGORY LOW   HIGH   UNITS   QUALITATIVE  
N/A N/A N/A N/A >= 0.400
   
Unit of measure: OD Units
Ideal sample: N/A
Absolute minimum amount: 0.2 mL serum
Method of collection: N/A
Transportation: N/A
Label instructions: N/A
The following substances can adversely affect the results: Samples collected in Heparin.  Microbially contaminated, hemolyzed, icteric, lipemic, or heat-inactivated sera may give inconsistent results.
Request test on form: Special Coagulation ( D6115 )
Order/entry screen(s): Hematology/Coagulation
Stat frequency: Once Daily, Monday-Friday
Stat turn around time: N/A
Routine frequency:  Once Daily, Monday-Friday
Must be in by: 11:00am
Routine turn around time: Varies with time of sample.  Assay is performed once a day after 11:00am and takes approximately 3 hours.
For Hillcrest deliver to: SCRAP : Specimen Central Receiving and
  Processing, room 2-117
For Thornton deliver to: Hematology : Processing Desk, 1st floor,
  Hospital, room 1-013
Performed at: Hillcrest Clinical Laboratories
Scheduling requirements : N/A
Method: Enzyme-Linked Immunosorbent Assay (ELISA)
Special Instructions: Serum should be separated from red cells.  Samples that are not tested immediately should be stored at 2-8C.  Samples that are not tested within 4 hours (collected after 11:00 M-F or on weekends)  Should be frozen.  Only whole human serum is suitable for this assay.  Although a positive reaction obtained using this assay may indicate the presence of a Heparin-associated antibody, the detection of such antibodies, however, does not confirm the diagnosis of Heparin-Induced Thrombocytopenia (HIT).  If clinically indicated, the HIPA assay (Heparin-Induced Platelet Aggregation) may be performed.  Contact the special coagulation laboratory for scheduling information. 619 543 2249.
  Results are available on a Chart Copy
  Results are Qualitative
CyberLAB abbreviation: HPF4
CyberLAB code: 6129
Lab Processing Instructions: See 'Special Instructions' above.