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  Workup for Rh Immunoglobulin Specimen Type:
  also known as Rhogam Workup, Rhogam

Blood



Specimen requirements: 6 mL Lavender Tube (EDTA)
Unit of measure: N/A
Ideal sample: 6 mL
Absolute minimum amount: 5 mL
Method of collection: Venipuncture
Transportation: N/A
Label instructions: Date, time, and legible signature by the responsible phlebotomist must be noted on the label.
The following substances can adversely affect the results: Hemolysis
Request test on form: MULTI LABS [Chem/Hem/UA/Tox/BB/Rheum]
Order/entry screen(s): Transfusion Services/Blood Bank
Stat frequency: N/A
Stat turn around time: N/A
Routine frequency: Daily
Must be in by: N/A
Routine turn around time: 8 Hours
For Hillcrest deliver to: Blood Bank : Blood Bank, room 2-103
For Thornton deliver to: Blood Bank : Processing Desk, 1st floor, Hospital, room 1-013
Scheduling requirements : N/A
Method: N/A
Special Instructions: No specimen is needed if blood bank has record of Rh typing (must be Rh negative) and recent antibody screen (must not already be sensitized to Rh(d) antigen). Indications: Rh immune globulin (anti-D IgG, or RhIg is administered IM to Rh(d) negative mothers at the 28th week of pregnancy, within 72 hours post-delivery of an Rh(d) positive infant, post-abortion, trauma, external fetal manipulation, invasive procedures involving the amniotic cavity, e.g. amniocentesis. It is also given to non-pregnant Rh(d) negative females < 50 years old after transfusion of a Rh(d) positive blood product that contains small numbers of red cells, such as platelets. For patients given repeated doses of platelets over a period of time, it will be necessary to give additional doses every 3 weeks, since the half-life of IgG is approximately 21 days. It is not necessary to give RhIg after transfusion of a cellular blood components, e.g. FFP or cryo and it is impractical to attempt to treat an individual who has received an entire unit of Rh-positive RBC. RhIg is not indicated in persons who are already immunized to the Rh(d) antigen. This product carries no risk of infectious disease. Dose: the standard 300 mg dose of Rh(d) immune globulin will protect against immunization by up to 15 mL packed red cells (approximately 25-30cc of whole blood), or up to 30 units of random platelets or 3 units of apheresis platelets. In Rh(d) negative women who give birth to Rh(d) positive infants, a quantitative test for the presence of fetal blood in maternal circulation should be performed (kleihauer-betke) by the blood bank to determine if a single dose of RhIG is sufficient to prevent immunization. The 72-hour treatment limit is arbitrary and treatment should not be withheld even if more than 72 hours have elapsed.
  Results are available in PCIS
CyberLAB abbreviation: N/A
CyberLAB code: N/A
Lab Processing Instructions: N/A