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  Routine Sputum Culture Specimen Type:
  also known as Sputum Culture Routine, Lukens-Trap, Induced Sputum, Respiratory Routine Culture



Specimen requirements: Sterile Specimen Container
  or: Sterile Trap (Lukens-Trap)
Unit of measure: N/A
Ideal sample: 2 -10 mL
Absolute minimum amount: 2 mL
Method of collection: Expectorated or induced sample
Transportation: Deliver to laboratory within 1 to 2 hours. Delayed samples should be kept at room temperature.
Label instructions: All containers must be labeled with patient name and medical record number.
Request test on form: Multi Labs/Micro/Viro/Serology ( 151-905 )
Order/entry screen(s): Microbiology/Virology
Stat frequency: N/A
Stat turn around time: N/A
Routine frequency: Maximum of 1 culture/24 hours.
Must be in by: N/A
Routine turn around time: 24 hrs for prelim/ 48-96 hrs for final
For Hillcrest deliver to: Microbiology : Room 2-109
For Thornton deliver to: Microbiology : Processing Desk, 1st floor,
  Hospital, room 1-013
Scheduling requirements : Specimens accepted in microbiology, Room 2-105, 24 hours/day.
Method: N/A
Special Instructions: Clinical significance of culture: to identify infectious organisms occurring within the respiratory system. Do not send specimen of saliva or spittle (unsatisfactory). If gram stain demonstrates more than 25 epithelial cells/lpf, the report will state: "saliva? Please resubmit". 1. Obtain sterile 4 oz. plastic specimen container from floor stock. 2. Prior to specimen collection, cleansing of the mouth is necessary. Instruct patient to wash mouth with chloraseptic x2 for 1 minute then rinse mouth with water. Do not cleanse mouth if specimen is for viral culture. 3. Instruct patient to expectorate first "deep" sputum specimen directly into container and to close top of container. 4. Submit specimen as soon as possible after collection. If specimen is delayed, then store at room temperature. A sample requested for viral culture should be refrigerated (see viral culture Information.)
  Results are available on a Chart Copy
  Results are Qualitative
SOFT LAB abbreviation: CXRES
Secondary code: N/A
Lab Processing Instructions: N/A