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  Cerebrospinal Fluid (CSF) Culture Specimen Type:
  also known as CSF Culture, Routine CSF Culture, Spinal Fluid Culture, Ventricular Fluid Culture, Lumbar Puncture

Spinal Fluid


Specimen requirements: Cerebrospinal Fluid Collection Tubes
  or: Syringe
  or: Sterile Snap Cap Tube (13 X 100)
Unit of measure: N/A
Ideal sample: 1.0 mL for routine culture
Absolute minimum amount: 0.5 mL for routine culture
Method of collection: Obtain by lumbar puncture using a spinal fluid needle.
Transportation: Submit tubes directly to the Microbiology Lab immediately. Do not refrig. unless viral cult requested.
Label instructions: All containers must be labeled with patient name and medical record number.
Request test on form: Multi Labs/Micro/Viro/Serology ( 151-905 )
Order/entry screen(s): Microbiology/Virology
Stat frequency: N/A
Stat turn around time: N/A
Routine frequency: Daily
Must be in by: N/A
Routine turn around time: 48 Hours for negative routine culture.
For Hillcrest deliver to: Microbiology : Room 2-109
For Thornton deliver to: Microbiology : Processing Desk, 1st floor,
  Hospital, room 1-013
Scheduling requirements : N/A
Method: N/A
Special Instructions: Place CSF into sterile leak proof tubes available in lumbar puncture trays. Make sure that tube closures do not create an aerosol when tubes are opened. Submit at least 3 tubes so that CSF can be analyzed in Chemistry, Hematology, and Microbiology. ***To avoid the potential for contamination of the material, all tubes must be delivered first to the Microbiology Laboratory*** Microbiology will record the description and volume of the fluid on the "cell count" requisition, take the appropriate tube(s) for culture or serology testing and give the remaining request slips and samples to the courier delivering the samples. The courier will take the appropriate samples and request forms to the SCRAP lab, and the processor will dispense the remaining samples to Chemistry, Hematology and Cytology ( if requested). If no specific indication has been made by the physician for the disposition of the numbered tubes, the specimen tubes will be processed in following manner: Tube #1 Chemistry Tube #2 Microbiology/Virology culture/Serology Tube #3 Hematology cell count Tube #4 Cytopathology. Submit a sufficient volume of fluid for culture: 1.0 mL for routine culture 1.0 mL for Serology tests 2.0 mL for AFB culture 1.0 mL for PCR procedures 1.0 mL for fungus culture 2.0 mL for virology culture. Only CSF for viral studies or PCR procedures should be refrigerated or frozen. Routine culture will include a gram stain, culture for aerobes.
  Results are available on a Chart Copy
SOFT LAB abbreviation: CXCSF
Secondary code: N/A
Lab Processing Instructions: N/A