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  Cerebrospinal Fluid AFB Culture Specimen Type:
  also known as CSF AFB Culture, AFB Culture-CSF, Acid Fast Culture CSF, Spinal Fluid AFB Culture, Ventricular Fluid AFB Culture, Lumbar Puncture fluid AFB Culture

Spinal Fluid


Specimen requirements: Cerebrospinal Fluid Collection Tubes
  or: Syringe
  or: Sterile Snap Cap Tube (13 X 100)
Unit of measure: N/A
Ideal sample: 2.0 mL
Absolute minimum amount: 1.0 mL
Method of collection: Obtain by lumbar puncture
Transportation: Transport to the Microbiology Laboratory immediately.
Label instructions: All containers must be labeled with patient name and medical record number.
Request test on form: Multi Labs/Micro/Viro/Serology ( 151-905 )
Order/entry screen(s): Microbiology/Virology
Stat frequency: Daily
Stat turn around time: Smear - 1 hour Culture - N/A
Routine frequency: Daily
Must be in by: N/A
Routine turn around time: 24 Hours for stain/ 8 weeks for culture
For Hillcrest deliver to: Microbiology : Room 2-109
For Thornton deliver to: Microbiology : Processing Desk, 1st floor,
  Hospital, room 1-013
Scheduling requirements : N/A
Method: N/A
Special Instructions: Place CSF into sterile leak proof tubes available in lumbar puncture trays. Make sure tube closures do not create an aerosol when the tubes are opened. Unless ordered for AFB culture only, submit at least three tubes of fluid to ensure adequate sample for analysis in Chemistry, Hematology, or for other bacteriological cultures. All tubes must be delivered first to the Microbiology laboratory to avoid the potential for contamination of the material. If ordered for tests other than AFB culture, the Microbiology laboratory will record the description and volume of the fluid on the "cell count" requisition; take the appropriate tube(s) for culture and give the remaining request slips and samples to the courier delivering the samples. The courier will take the samples and requestcards to the SCRAP lab processor who will disburse the remaining samples to the Chemistry, Hematology, and Cytology laboratories.
  Results are available on a Chart Copy
SOFT LAB abbreviation: CXAFB
Secondary code: 4601
Lab Processing Instructions: N/A