|WELCOME TO UCSD LABORATORY SERVICES GUIDE|
|# A B C D E F G H I J K L M N O P Q R S T U V W X Y Z|
|Electron Microscopy||Specimen Type:|
|Unit of measure:||N/A|
|Absolute minimum amount:||N/A|
|Method of collection:||N/A|
|Label instructions:||Specimen must be labeled with patient name, Medical Record and Patient numbers.|
|Request test on form:||Surgical Pathology/Neuropathology Consult Request ( 151-025 )|
|Stat turn around time:||N/A|
|Must be in by:||N/A|
|Routine turn around time:||3-5 Days|
|For Hillcrest deliver to:||Surgical Pathology : 2nd Floor, Tissue Gross|
|Deliver the specimen to :||NA : NA|
|Scheduling requirements :||N/A|
|Method:||Electron Microscopy Methods:
The "request for Surgical Pathology / Neuropathology consultation" (white, 9 1/2 x 11, form 151-025) must be filled out completely before submitting the specimen to the laboratory. It is the responsibility of the operating physician to complete this form. If the patient has had pertinent tissue examined previously at another hospital, please notify the Surgical Pathology office (ext 35764 at hillcrest, ext 76613 at Thornton) so that these slides can be obtained. It is a hospital accreditation requirement that these slides be obtained and reviewed.
Both tissue and fluids should be placed immediately upon removal into Karnovsky’s fixative (2% paraformaldehyde + 2.5% glutaraldehyde in 0.1M Phosphate Buffer). Volume of fixative should be at least 10 times that of the specimen. The fixative must be fresh because it oxidizes rapidly and becomes ineffective.
Large tissue specimens should be trimmed to approximately 0.5-1.0 cm blocks with a thickness no greater than 0.4 cm. However, the tissue should not be “minced” into smaller blocks as this introduces artifact.
Fresh fixative is available from the gross room in Room 2-304. Questions regarding specimen fixation and handling can be referred to the EM lab at 543-6167 or to the neuropathology resident at pager 290-5545. The regular working hours are Monday-Friday 800-1630. For questions after hours, please call the page operator at 543-6040 and ask for the neuropathologist on-call.
|Results are available on a Chart Copy|
|Lab Processing Instructions:||N/A|