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  Pertussis PCR  - (sendout) Specimen Type:



Specimen requirements: 1.  Two flexible wire Dacron-tipped swabs (Dacroswab, type 3)  Do not use wood, plastic, or calcium alginate swabs (Calgiswab).
2.  Sterile screw-capped tube containing 0.5 mL of sterile physiological saline, water, or phosphate buffered saline (PBS).
Unit of measure: N/A
Ideal sample: 2 Swabs (not wooden applicator sticks).  Available from Microbiology
Absolute minimum amount: 1 Swab
Method of collection: 1.  Carefully insert a flexible-wire Dacron-tipped swab through one nostril into the posterior nasopharynx and rotate the swab.  Keep the swab near the septum and floor of the nose.
2.  Place the entire swab into the transport tube, immersing the tip into the fluid.
3.  Repeat the collection steps using a second swab for the other nostril and place the swab
     into the same tube.
4.  Cap the tube and label with first and last names, DOB, or other unique identifier.    
Transportation: Transport to the specimen on ice to the laboratory ASAP.  Store at -70C.
Label instructions: N/A
Request test on form: Multi Labs/Micro/Viro/Serology ( 151-905 )
Order/entry screen(s): Microbiology/Virology
Stat frequency: N/A
Stat turn around time: N/A
Must be in by: 0830 for same day results. M, W, F
Routine turn around time: variable (8 hours to 48 hours)
Deliver the specimen to : Microbiology
This test is sent to : Children's Hospital
Scheduling requirements : N/A
Method: Polymerase Chain Reaction
Special Instructions: N/A
  Results are available on a Chart Copy
  Results are Quantitative
CyberLAB abbreviation: BPPCR
CyberLAB code: 9607
Lab Processing Instructions: N/A