Researchers Identify microRNA Targets in C. elegans

Regulating protein levels is key to biomedical research in humans and model organisms

January 11, 2010  |  

MicroRNAs (miRNAs) are non-coding RNAs that impact almost every aspect of biology.  In recent years, they have been strongly implicated in stem cell biology, tissue and organism development, as well as human conditions ranging from mental disorders to cancer. For the most part, miRNAs control gene expression of messenger RNA (mRNA) targets.  Unlike mRNAs, which are translated into proteins, miRNAs function as short, untranslated molecules that regulate specific mRNAs through base-pairing interactions. Since miRNAs bind limited stretches of consecutive bases in mRNAs, identifying which mRNAs are targets of individual miRNAs has been a bottleneck of biomedical research, as researchers have had to rely largely on computational predictions.

Now, researchers at the University of California, San Diego have identified the binding sites of these miRNAs in one of the foremost model organisms, C. elegans, using biochemical means to capture targeted mRNA sequences in vivo.

Argonaute proteins are key players in gene-silencing pathways; miRNAs are anchored into specific binding sites to guide Argonaute proteins to target mRNA molecules for silencing or destruction. By cross-linking interactions between the Argonaute protein bound to miRNA and mRNA duplexes, principal investigators Gene Yeo, PhD, assistant professor in UCSD’s Department of Cellular and Molecular Medicine and Amy Pasquinelli, PhD, associate professor in UCSD's Division of Biological Sciences, were able to globally identify their specific binding sites in the nematode.

“Our results were very surprising in that we discovered that individual miRNAs can interact with their targets very differently, and differently than we had expected,” said Yeo.  “This approach, and the computational analyses that were develop, open up new ways to identify individual miRNA targets in any tissue and cell type in almost any organism."

"The revelation of thousands of endogenous miRNA target sites provides an unprecedented wealth of data for understanding how miRNAs regulate specific targets in a developing animal," added Pasquinelli. 

Their work was published online in advance of print on January 10 by Nature Structural & Molecular Biology.

# # #

Media Contact: Debra Kain, 619-543-6163, ddkain@ucsd.edu




Media Contact

Share This Article


Related News

11/20/2017
The UC San Diego Health International Patient Program was recognized as the 2017 Medical Provider of the Year by the International Travel & Health Insurance Journal (ITIJ). ITIJ recognizes leading hea ...
11/16/2017
That “to do” list of chores and errands could actually provide a variety of health benefits, according to researchers at the University of California San Diego School of Medicine. The study, published ...
11/15/2017
UC San Diego Health has expanded its treatment of rare brain tumors by launching a specialized program in the diagnosis and treatment of acoustic neuromas and complex skull base tumors. The new progra ...
11/14/2017
In an unprecedented move, UC San Diego Health and UCI Health have formed a strategic partnership to increase operational efficiencies and decrease patient care costs by sharing a single electronic med ...



Follow Us